Asus AAM6010EV-M User Manual download pdf (Page 5)



Ispitivanje direktne antibakterijske

aktivnosti prolinom-bogatih peptida, galarmina

i analoga, na meticilin-rezistentni Stalokokus

aureus i klinički značajne Gram-pozitivne i

Gram-negativne bakterije, in vitro

ORIGINALNI RAD / ORIGINAL ARTICLE

Investigation of direct antibacte-

rial activity of proline-rich pep-

tides, galarmin and analogues,

against methicillin-resistant

Staphylococcus aureus and clini-

cally relevant Gram-positive and

Gram-negative bacteria, in vitro

Andranik A. Durgaryan









PRIMLJEN / RECEIVED 10.10.2010

PRIHVAĆEN / ACCEPTED 22.11.2010

KORESPONDENCIJA / CORRESPONDENCE





strana / page 131





APSTRAKT

Cilj. Prolinom bogati polipeptidi, galarmin i analozi, su

novi neuropeptidi koje je u neurosekretornim granulama

hipotalamusa otkrio i izolovao prof. A. Galoyan sa sarad-

nicima. Pokazano je da galarmin i analozi imaju veliku

biološku aktivnost uključujući snažnu in vivo imunomod-

ulatornu i antibakterijsku aktivnost protiv velikog broja

dobro poznatih patogena. Cilj ove studije je bio da se ispita

direktna antibakterijska aktivnost galarmina i analoga pro-

tiv različitih Gram-pozitivnih i Gram-negativnih bakterija

uključujući MRSA sojeve in vitro.

Metod. Direktna antibakterijska aktivnost galarmina i

analoga je odredjivana esejom inhibicije rasta u tečnosti

bakterija protiv različitih sojeva. Bakterije su gajene u Lu-

rija-Bertani standardnom tečnom medijumu tokom 2-3h

na 37°C, 250 o/min. Minimalne inhibitorne koncentracije

(MIC) za galarmin i analoge su utvrđene merenjem apsor-

pcije (optičke gustine - OG) udubljena u ploči, na 630 nm,

pomoću čitača ploča (Asys UVM 340). Statistička analiza

svih podataka izvršena je Studentovim i Fišerovim testom,

pri verovatnoći od p≤0.05.

ABSTRACT

Objective. Proline-rich polypeptides, galarmin and ana-

logues, are new neuropeptides discovered and isolated from

neurosecretory granules of hypothalamus by Prof. A. Galoyan

and coworkers. It was shown that galarmin and analogues

have large biological activity including strong immunomodu-

latory and antibacterial activity in vivo against a number of

well knows pathogen. The purpose of this study was to inves-

tigate the direct antibacterial activity in vitro of galarmin and

analogues against different Gram-positive and Gram-negative

bacteria, including MRSA strains.

Method. Direct antibacterial activity of galarmin and its

analogues was monitored by a liquid growth inhibition assay

against different bacterial strains, Bacteria were cultivated in

Luria-Bertani standard liquid medium during 2-3h at 37°C, 250

rpm. The minimal inhibitory concentrations (MICs) of galar-

min and analogues were determined by measuring the absor-

bance (optical density - OD) of the plate’s wells at 630 nm by

plate automatic reader (Asys UVM 340). Statistical analysis of

all data was performed by Student’s and Fishers’ methods, at

probability of p≤0.05.

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